Time Lapse: Growth and 3D Segmentation of WT, bri1, and BL Treated Arabidopsis Roots

Original video: https://youtu.be/KoqLgKzarfQ

Part 1:

Shows a time-lapse of root growth in wild-type (WT), bri1 mutant, and brassinolide (BL)-treated Arabidopsis seedlings. The roots express the 35S–Lti6b–eGFP marker and were imaged using confocal microscopy every 30 minutes for a total of 6 hours. A Standard Deviation (STD) Z-projection of the root is presented, allowing visualization of growth dynamics. This video was used to quantify meristematic cell displacement.

Part 2:

Presents a 3D visualisation of root growth in wild-type (WT), bri1 mutant, and brassinolide (BL)-treated Arabidopsis seedlings. Roots were imaged over time (WT: 13 hours, bri1: 10.5 hours, BL-treated: 12 hours), segmented in 3D, and the initial and final states were morphed to depict root growth. The video clearly distinguishes the elongation and meristem zones, providing a visual representation of root development over time.

Keywords: Arabidopsis, Root Growth, Elongation, Confocal microscopy, 35S–Lti6b–eGFP, Meristematic Cell Displacement, 3D segmentation, Time-lapse

Citation:

Fridman, Y., Strauss, S., Horev, G. et al. The root meristem is shaped by brassinosteroid control of cell geometry. Nat. Plants 7, 1475–1484 (2021). https://doi.org/10.1038/s41477-021-01014-9

Published: 15 November 2021

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