Time-Lapse: Asymmetric Neuroblast Divisions in the Drosophila wL3 Brain Lobe 🧠🔬
- PlantHouse Enterprise
- 20 hours ago
- 1 min read
Published on: 06 October 2020
Witness the dynamic process of neurogenesis in this high-resolution 2-photon time-lapse of a wandering third-instar (wL3) Drosophila larval brain lobe. Using a specialized agarose immobilization technique, the explanted tissue remains perfectly stable for long-term imaging without the use of harsh adhesives or physical compression.In this sequence.
Neuroblasts are clearly identified by their significantly larger cell size compared to surrounding cells. You can observe these neural stem cells undergoing asymmetric divisions to produce Ganglion Mother Cells (GMCs), which are also highlighted as they proceed through their own subsequent divisions. Chromosomal movement and mitotic phases are visualized through the His2Av::eGFP marker, providing a clear view of the genetic material during every stage of the cell cycle.
Keywords:
Neuroblast Division, Drosophila Melanogaster, 2-Photon Imaging, Larval Brain, His2Av::eGFP, Cell Biology, Asymmetric Division, Ganglion Mother Cell, Neurogenesis, Time-lapse Microscopy, Frontiers in Cell and Developmental Biology.
Citation:
Bostock MP, Prasad AR, Chaouni R, Yuen AC, Sousa-Nunes R, Amoyel M and Fernandes VM (2020) An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues. Front. Cell Dev. Biol. 8:590094. doi: 10.3389/fcell.2020.590094
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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