Time-Lapse: Neuroblasts Reactivating from Quiescence in Drosophila VNC 🔬🧠
- PlantHouse Enterprise
- 17 minutes ago
- 1 min read
Published on: 06 October 2020
Witness the precise moment of cellular awakening in this 2-photon time-lapse of neuroblasts reactivating from quiescence. Filmed using an advanced immobilization technique, this sequence captures the late first-instar (L1) Ventral Nerve Cord (VNC) of a Drosophila larva in a lateral orientation—the optimal view for visualizing delicate neuroblast fibers.
The video begins 24 hours after larval hatching, a period when the majority of VNC neuroblasts are in a quiescent state. These neural stem cells are labeled with grh> syn21-GFP-p10. As the time-lapse progresses, you can observe the first reactivation divisions occurring at approximately 01:40:00. This high-resolution footage provides a rare look at the fine cellular processes and structural changes that occur as stem cells transition back into the cell cycle.
Keywords:
Neuroblast Reactivation, Quiescence, Ventral Nerve Cord, Drosophila Larva, 2-Photon Microscopy, Cell Biology, Neural Stem Cells, GFP Labeling, Time-lapse Microscopy, Frontiers in Cell and Developmental Biology.
Citation:
Bostock MP, Prasad AR, Chaouni R, Yuen AC, Sousa-Nunes R, Amoyel M and Fernandes VM (2020) An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues. Front. Cell Dev. Biol. 8:590094. doi: 10.3389/fcell.2020.590094
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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