Three-Dimension Reconstruction of Tobacco Anther Meiocytes: Unveiling Pachytene Nuclear Dynamics

Original video: https://youtu.be/jtDh_8xd_KQ This video presents a stunning 3D reconstruction of male meiocytes at the pachytene stage within a tobacco anther. Created from an impressive series of 2,626 serial images, this visualisation allows us to observe the complex spatial arrangement and dynamic behavior of nuclei during this critical stage of meiosis. Migrating nuclei are highlighted in yellow, non-migrating nuclei in red, partially scanned nuclei in blue, and the outer cell wall layer in turquoise (labeled on every 50th slice). Witness the power of 3D reconstruction in unraveling the intricacies of plant reproductive biology.

Keywords: 3D, Volume electron microscopy (vEM), Male meiosis, Tobacco, Anther, Nuclear Migration

Citation:

Mursalimov S, Ohno N, Matsumoto M, Bayborodin S and Deineko E (2021) Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes. Front. Plant Sci. 12:672642. doi: 10.3389/fpls.2021.672642

Received: 26 February 2021; Accepted: 07 April 2021;

Published: 07 May 2021.

Attribution 4.0 International — CC BY 4.0 - Creative Commons