Original video: https://www.youtube.com/watch?v=TuFl_ZHitwk
In this video, we present a developmental series of Antirrhinum majus (Snapdragon) buds and flowers, visualized through high-resolution fluorescent and transmission OPT scans. The series captures bud growth stages, ranging from 0.5 mm to a fully mature flower, using distinct imaging resolutions and methods to highlight structural changes.
The smallest buds, measuring 0.5 to 1.2 mm, were scanned with a GFP1 filter on the MRC OPT scanner to capture fluorescent emissions, revealing detailed internal structures. Bud 1 is shown with a high scan resolution of 3 µm per voxel, while Bud 2 is scanned at 4.8 µm per voxel.
For the slightly larger buds, between 2.5 mm and 7 mm in depth and up to 10 mm in height, transmission OPT scans were conducted using the Bioptonics 3001 OPT scanner. Bud 3 is shown at a resolution of 8.2 µm per voxel, and Bud 4 at 24.4 µm per voxel.
Finally, a mature flower with dimensions of 15 mm in depth and 40 mm in height underwent an M-OPT emission scan using a GFP1 filter with a resolution of 50.4 µm per voxel. This view highlights the complex floral anatomy at full maturity, illustrating the growth progression from a bud to a fully developed flower. This developmental series provides insight into the structural evolution of Snapdragon flowers, using OPT imaging to capture the intricate details across different stages.
Keywords: 3D, development, gene activity, imaging, macroscopic, macro optical projection tomography, M-OPT, plant-insect interactions, tomography, whole plant.
Citation: Karen J. I. Lee, Grant M. Calder, Christopher R. Hindle, Jacob L. Newman, Simon N. Robinson, Jerome J. H. Y. Avondo, Enrico S. Coen, Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity, Journal of Experimental Botany, Volume 68, Issue 3, 1 January 2017, Pages 527–538, https://doi.org/10.1093/jxb/erw452
Published: 26 December 2016
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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