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How to Count and Measure Roots Using ImageJ: A Protocol for Auxin Experiments
https://youtu.be/PqQIvCWd6Dg Summary Technical training session established standardised ImageJ measurement protocols for quantifying auxin experiment root growth and length. ImageJ Setup and Scaling: Participants installed the ImageJ software and configured image scaling by setting rulers to establish consistent pixel-to-millimetre units. Counting and Measuring Roots: Researchers utilised multi-point tools for root counting and freehand tracing for curvature measurement. Ea
Scense Me
Apr 306 min read
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Practical Plant Growth Analysis Galangal Plastochron
1. Introduction Growth analysis is a mathematical expression of environmental effects on the growth and development of crop plants. Classical growth analysis typically requires the destructive harvesting of representative sets of plants over several weeks, making it impossible to follow the same plant throughout an entire experiment. This practical introduces a non-destructive, single-harvest alternative by utilizing a space-for-time substitution. By examining a single frond
Scense Me
Apr 204 min read
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Practical-Auxin: Investigating Root Initiation in Alternanthera sissoo (Bayam Brazil)
Introduction & Theory Plant propagation through stem cuttings is a fast and cost-effective technique that maintains the genetic traits of the parent plant. The success of this method depends heavily on the rapid formation of adventitious roots. This process is regulated by plant hormones called auxins, which stimulate cell division in the stem's vascular tissues. Indole-3-butyric acid (IBA) is a widely used synthetic auxin because of its stability and effectiveness across man
Scense Me
Apr 203 min read
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Practical-Psi: Investigating Water Potential of Potato Tissue
🎯 Aim To determine the water potential of potato tissue by immersing potato cylinders in sucrose solutions of different concentrations and measuring the percentage change in mass. 🧰 Materials Fresh potato Cork borer (to cut uniform circular cylinders) Scalpel and ruler (to trim equal lengths) Balance (±0.01 g) Blotting paper/tissue 6 labelled beakers or test tubes 50 mL each of sucrose solutions: 0.0 M, 0.1 M, 0.2 M, 0.3 M, 0.4 M, 0.5 M Timer Graph paper or excel spreadshee
Scense Me
Apr 132 min read
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Crop Physiology Practical Determination of Leaf Area
Original video: https://youtu.be/QXD5rnNXvfg Lecture on: 22 October 2025 Welcome to the essential guide on determining leaf area—a critical measurement in plant physiology and crop growth analysis! Accurate leaf area data is vital for calculating parameters like Leaf Area Index (LAI) and Net Assimilation Rate (NAR). In this video, we guide you through the setup and procedure for three common methods used to measure leaf area in the laboratory: 1. Graph Paper Method (Manual):
PlantHouse Enterprise
Nov 16, 20251 min read
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