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AGR3301 Crop Physiology: Crop Growth Analysis Experiment Manual

Updated: Apr 1



 Download Easy Leaf Area app here: Full lab manual


🌱 Overview

This experiment studies how different nutrient solutions affect the growth of duckweed (Lemna sp.), a fast-growing aquatic

plant.


You will measure plant growth using:

  • Leaf Area

  • Biomass (fresh & dry weight)

  • Growth analysis indices (LAI, CGR, NAR, AGR, RGR)


🎯 Objective

  • Compare duckweed growth under different nutrient solutions


🧪 Treatments

  • Each group is assigned ONE treatment (1% solution):T1 – Blood MealT2 – Feather MealT3 – UreaT4 – CIRPT5 – White SugarT6 – Melaka Palm Sugar


📦 Basic Setup

  • - 6 cups per group- 3 fronds per cup- 150 mL nutrient solution- Place in bright area (NO direct midday sun)

🏷️ Labelling System

  • Day 5 (T1):R1-T1, R2-T1, R3-T1Day 10 (T2):R1-T2, R2-T2, R3-T2


🔁 Daily Care (Day 1–10)

  • Keep in bright light- Top up nutrient solution every 2–3 days- Do NOT change treatment solution


📊 Data Collection (Day 1, 5, 10)

1. Light Measurement

  • Use GPS Status App → record in Lux


2. Leaf Area Imaging

  • Use Easy Leaf Area App- Place 2 cm × 2 cm red scale- Take aerial photo


3. File Naming:

  • Treatment-Day-ReplicationExample: Urea-R1-T1

  • Record the leaf area value in the provided excel link: Communal excel data spreadsheet.xlsx. Create your own group sheet in the excel.

  • Use the phone camera, take the one aerial image and save your image name using the format: Treatment-Day-Replication (e.g., BloodMeal-R1-T1).

  • Upload: Upload the daily images to the dedicated group folder: STUDENT SPACE AGR33001 SEM2 MAC-JULY 26


⚗️ Harvest (IMPORTANT)

Day 5:

Harvest R1–R3Measure:- Leaf Area- Fresh Weight- Dry Weight (after oven)

Day 10:

Repeat for remaining samples


📐 Key Calculations (SIMPLIFIED)

LAI

LAI = Leaf Area / Ground Area

CGR

CGR = (W2 - W1) / (t2 - t1) / GA

NAR

NAR = (W2 - W1) / (t2 - t1) × [ ln(LA2) - ln(LA1) ] / (LA2 - LA1)

AGR

AGR = (W2 - W1) / (t2 - t1)

RGR

RGR = [ ln(W2) - ln(W1) ] / (t2 - t1)


📢 Final Note

Each group only has ONE treatment.You MUST combine data with other groups to:- Compare all 6 treatments- Complete your analysis

 

 

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