A Long Term Time Lapse Microscopy Technique For Arabidopsis Roots
- PlantHouse Enterprise
- 19 hours ago
- 1 min read
Published on: 09 June 2025
This video demonstrates a robust workflow for long-term, high-resolution live imaging of plant roots using the Mizar TILT light sheet system.
The first segment details the Seedling Mounting Procedure, illustrating a step-by-step "sandwich" technique. By positioning seedlings between a low-melt agarose blanket and a chambered cover glass, researchers can maintain the root in a stable imaging plane while allowing for natural growth and gas exchange.
The second segment presents the Experimental Result: a 24-hour time-lapse of a regenerating Arabidopsis root following laser ablation. Using three fluorescent reporters (35S:H2B-RFP, WOX5:erCFP, and PET111:YFP), the video captures the dynamic re-establishment of the Quiescent Center (QC) and the reorganization of the WOX5 expression domain in real-time.
Keywords: Microscopy, Time lapse microscopy, Root development, Light sheet microscope, Regeneration.
Citation:
Lee LR, Rahni R and Birnbaum KD (2025) A long term time lapse microscopy technique for Arabidopsis roots. Front. Plant Sci. 16:1601397. doi: 10.3389/fpls.2025.1601397
Attribution 4.0 International — CC BY 4.0 - Creative Commons
Comments