Original video: https://www.youtube.com/watch?v=B6QddljTgh0
This video demonstrates the process of manually assigning parent labels to inner epidermal cells in Arabidopsis using MorphoGraphX. We begin by loading the inner epidermal meshes from two time points: T1 (24-hour) and T2 (48-hour). To assign parent labels, the T1 mesh is hidden, leaving only the segmented cell outlines visible, while the T2 mesh remains active with parent cells highlighted. The T1 mesh is then overlaid onto the T2 mesh, allowing parent cells to be matched with their corresponding daughter cells. Parent labels are manually assigned by clicking within the parent cell regions.
Next, we show how to track cell lineages over time using the 'Parent Tracking' process in MorphoGraphX, which links daughter cell labels in the T2 mesh with their respective parent cell labels in the T1 mesh. The segmented T1 and T2 meshes are loaded into separate stacks, with distinct colors assigned to avoid confusion. Manually labeled parent cells are used as landmarks for automatic parent labeling, simplifying the process by auto-labeling the remaining daughter cells.
The video highlights key steps such as superimposing meshes and using the 'Grab label from other surface' tool to transfer parent labels, ensuring a smooth and accurate tracking of cell growth and division across multiple time points.
Keywords: growth, 2.5D segmentation, live imaging, image processing, deep tissue imaging, Arabidopsis, MorphoGraphX, sepals
Citation: Singh Yadav A and Roeder AHK (2024) An optimized live imaging and multiple cell layer growth analysis approach using Arabidopsis sepals. Front. Plant Sci. 15:1449195. doi: 10.3389/fpls.2024.1449195
Received: 14 June 2024; Accepted: 29 July 2024;
Published: 03 September 2024.
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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