Original video: https://www.youtube.com/watch?v=VGoRU1VQWcY
In this video, we present a 3D visualization of Thrip (Thysanoptera) populations inside a snapdragon (Antirrhinum majus) flower, captured using Macro Optical Projection Tomography (M-OPT). Two M-OPT channels are combined to showcase various structures within the flower. Anthers, veins, and sepals are imaged in yellow using emission M-OPT, highlighting tissue autofluorescence via a GFP1 filter. Petals are shown in purple, while the thrips are visualized in turquoise using transmission M-OPT, all at a scan resolution of 40 µm/voxel.
A close-up zoom reveals a thrip located near the floral veins, surrounded by trichome hairs, with increased detail captured at a higher scan resolution of 6.5 µm/voxel. This detailed imaging highlights the intricate relationship between the insect and the floral structures.
Keywords: 3D, development, gene activity, imaging, macroscopic, macro optical projection tomography, M-OPT, plant-insect interactions, tomography, whole plant.
Citation: Karen J. I. Lee, Grant M. Calder, Christopher R. Hindle, Jacob L. Newman, Simon N. Robinson, Jerome J. H. Y. Avondo, Enrico S. Coen, Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity, Journal of Experimental Botany, Volume 68, Issue 3, 1 January 2017, Pages 527–538, https://doi.org/10.1093/jxb/erw452
Published: 26 December 2016
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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